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Proteomics Services

Amplify Proteins, Catalyze Expression, Fuel Discoveries

​Operon Technologies offers specialized protein analysis services to accelerate your scientific exploration. Our state-of-the-art techniques empower researchers and industries to delve into protein identification, characterization, quantification, and more. With a focus on precision and innovation, Operon Technologies is your partner in unraveling the mysteries of proteins and driving breakthrough discoveries.

Operon Technologies leads in proteomic services, unveiling hidden protein intricacies for pioneering discoveries. From identification to interactions, our advanced techniques empower researchers and industries to decipher proteins' roles. Partner with us to elevate your proteomic exploration and drive transformative insights.

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Key Protein Services offered by Operon Technologies:

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  • Protein Expression

    • Expression in bacterial hosts

    • Expression in Saccharomyces cerevisiae

    • Expression in Pichia Pastoris

  • Protein Purification

    • Affinity tag-based purification

    • Ion exchange-based purification

    • Hydrophobic Interaction based purification

    • Size exclusion chromatography

    • Scale up to 1g of purified protein

  • Protein Analysis

    • Electrophoresis

    • HPLC analysis

    • Activity assay

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  • Polyacrylamide Gel Electrophoresis (PAGE)

    • Native-PAGE

    • SDS-PAGE

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  • Iso-Electric Focusing (IEF)

    • Separation on Polyacrylamide IEF gels (Invitrogen)

    •  Separation on Immobilized pH gradient (IPG) strips (Biorad/ GE Healthcare)​

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  • Two – Dimensional Gel Electrophoresis (2 DE)

    • Gel based separation of complex protein mixtures based on pI and molecular weight

    • Expression analysis of protein spots using Image Master 2D Platinum-7 software (GE Healthcare)

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  • High Performance Liquid Chromatography (HPLC)

    • Purity Check

    • Free and Bound Amino Acid analysis - Reverse Phase

    • HPLC (RP-UPLC and Cation Exchange -HPLC)

    • Size Exclusion HPLC

    • Strong Cation Exchange HPLC

  • Fast Protein Liquid Chromatography (FPLC)

    • Purification of desired proteins Instrumentation

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  • Circular Dichroism (CD) Spectroscopy

    • Near and Far UV Spectra

    • Secondary structure determination

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  • Mass Spectrometry

    • MALDI (Matrix Assisted Lazer Desorption and Ionization)

    • LC-MS (Liquid Chromatography Mass Spectrometry)

    • GC-MS ( Gas Chromatography Mass Spectrometry)

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